期刊
EXPERIMENTAL PARASITOLOGY
卷 212, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2020.107886
关键词
Mesanophrys sp.; Portunus trituberculatus; Formalin; Toxicity; Prevent
类别
资金
- Major projects of Ningbo [2017C110007]
- China Agriculture Research System-48 [CARS-48]
- Talent Training Base of Agricultural Science and Education Cooperation, Ningbo University - Zhejiang Hongye Seafood Co., Ltd. [202000305]
- K.C.Wong Magna Fund in Ningbo University
In aquaculture of the swimming crab Portunus trituberculatus, massive deaths have been recorded in the winter months due to infection with a novel emerging parasite, Mesanophrys sp. However, no information was available regarding the prevention and control of this particular parasite. Therefore, the present study was conducted to evaluate the anti-parasitic efficacy and toxicity of formalin against the Mesanophrys sp. In vitro results showed that the anti-parasitic efficacy of formalin improved with concentration increasing from 0.0 to 20.0 ppm within 24 h. In particular, when treated with formalin at 16.0, 15.0, 11.0, 10.0, 9.0, and 6.0 ppm for 0.5, 1, 2, 4, 6, 12, and 24 h respectively, the Mesanophrys sp. mortality rate reached 100%. To gain insights into the effects the formalin treatment had on the parasite, cell micro- and ultra-structure were investigated. It was determined that the cells contracted gradually and became rounded, intracellular vacuoles were observed at early time points (Phi <= 4.83 +/- 1.26 mu m) and then disappeared. Cilia were shed and macronuclear chromatin became condensed and agglutinated. Small holes and bubbles appeared on surface of the parasites. In an in vivo trial, formalin was applied prior to Mesanophrys sp. artificial infection as prophylaxis to P. trituberculatus. The results showed that formalin prophylactic treatment effectively prevented P. trituberculatus from Mesanophrys sp. infection, thus remarkably reducing the mortality of crabs compared with the non-formalin-exposed and infected crabs. Furthermore, the normal behavior and survival of P. trituberculatus were not impacted by the prophylactic treatment.
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