期刊
CELLULAR SIGNALLING
卷 28, 期 9, 页码 1304-1313出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2016.06.010
关键词
NIMA-related expressed kinase 2A; Suppressor of fused; Phosphorylation; Transcription factor Gli2; Hedgehog signaling pathway
类别
资金
- China National Basic Research Program [2010CB535001]
- National Natural Science Foundation of China [31171359, 31460305, 81472607, 81560457]
- Natural Science Foundation of Jiangxi Province [20151BAB205037]
Suppressor of Fused (SuFu) plays a conservative role in the regulation of the Gli transcription factors within the Hedgehog (Hh) signaling pathway. Despite the central importance of SuFu in the Hh pathway, little is known about its regulation. Here, we performed a GAL4-based yeast two-hybrid screen using human SuFu as bait, and identified NIMA-related expressed kinase 2A (Nek2A) as a new SuFu-interacting protein, which was also confirmed by glutathione-S-transferase pull-down and co-immunoprecipitation assays. Intriguingly, Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein. Moreover, the phosphorylated SuFu inhibits the nuclear localization and transcriptional activity of Gli2/Hh signaling. These findings reveal a new mechanism of mammalian SuFu regulation, and offers novel insights into Hh signaling regulation in development and human disease. (C) 2016 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据