期刊
CELLULAR SIGNALLING
卷 28, 期 9, 页码 1292-1303出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2016.05.017
关键词
Porphyromonas gingivalis; Macrophages; NF-kappa B; ERK1/2; CD36; Ox-LDL
类别
资金
- Science and Technology Commission of Shanghai Municipality [13ZR1435600]
CD36, a scavenger receptor, plays an important role in the progression of atherosclerosis through its interaction with oxidized low-density lipoprotein (ox-LDL). Porphyromonas gingivalis (P. gingivalis, Pg) has been shown to promote macrophage-derived foam cell formation by affecting the expression of CD36. However, the regulatory role of CD36 in macrophages infected with Pg remains largely unknown. Therefore, the aim of the present study was to explore the molecular mechanism of Pg induced CD36 expression in macrophages. Our results showed that Pg promoted ox-LDL uptake by macrophages and the formation of foam cells. Pg infection increased CD36 mRNA and protein levels in ox-LDL-untreated macrophages. Moreover, small interferon RNA (siRNA) targeting CD36 significantly reduced foam cell formation induced by Pg. Additionally, Pg stimulated nuclear translocation of p65, which directly bound to the promoters of CD36 to facilitate its transcription. Inhibition of p65, NF-kappa B or ERK1/2 blocked Pg-induced CD36 production; whereas, overexpression of NF-kappa B subunits p65 and p50 upregulated CD36. Furthermore, Ras inhibitors significantly attenuated ERK1/2 activation and CD36 expression. Taken together, the data indicated that stimulation of the ERK/NF-kappa B pathway by Pg led to transactivation of the CD36 promoters, thereby upregulating CD36 expression in the infected macrophages. These findings may help design new treatment strategies in atherosclerosis. (C) 2016 Elsevier Inc. All rights reserved.
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