期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 40, 期 6, 页码 1559-1569出版社
KARGER
DOI: 10.1159/000453206
关键词
Bufalin; hTERT; CPSF4; Proliferation; Apoptosis; Migration
资金
- National Natural Science Foundation of China [81173615, 81472178]
- Science and technique support plane of the First Affiliated Hospital of Dalian Medical University [2013D005]
- State 973 Program of China [2014CB542005]
- Education Department of Liaoning Province in China (the Program for Pan-Deng Scholars)
- National Natural Science Foundation of Liaoning Province in China
Background/Aims: Bufalin can induce apoptosis in certain human cancer cell lines, but bufalin has not yet been thoroughly evaluated in colorectal cancer cells. Cleavage and polyadenylation specific factor 4 (CPSF4) and human telomerase reverse transcriptase (hTERT) play important roles in colorectal cancer growth. The aim of this study was to investigate the roles and interactions of bufalin, CPSF4 and hTERT and the effects of bufalin in human colorectal cancer. Methods: We treated LoVo and SW620 cells with bufalin to investigate the effect of bufalin on proliferation, apoptosis and migration. We verified the relationship between CPSF4 and hTERT using pulldown assays, luciferase reporter assays and chromatin immunoprecipitation (ChIP) assays. Results: Bufalin inhibited the proliferation and migration of and induced apoptosis in LoVo and SW620 cells. We identified CPSF4 as an hTERT promoter-binding protein in colorectal cancer cells. Conclusion: Our study identified bufalin as a potential small molecule inhibitor for cancer therapy. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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