期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 40, 期 5, 页码 883-894出版社
KARGER
DOI: 10.1159/000453147
关键词
TNF-alpha; Apoptosis; Autophagy; Cartilage degradation; miRNA-30b; ATDC5 cells
资金
- National Natural Science Foundation of China [81372000]
- Shanghai Jiao Tong University Medical-Engineering Cross Fund [YG2013MS25]
Objective: Cell death plays an important role in the pathology associated with inflammatory diseases such as osteoarthritis. It has been reported that autophagy can protect cells against tumour necrosis factor-alpha (TNF-alpha)-induced apoptosis. This study aimed to determine the potential role of microRNA-30b (miR-30b) in TNF-alpha-induced apoptosis, autophagy and differentiation in the chondrogenic ADTC5 cell line. Methods: To analyse the effect of TNF-alpha on the viability of ADTC5 cells, cell counting kit-8 and Hoechst 33342 staining were employed and the expression levels of caspase-3 and -9 were assessed. Autophagy was examined by analysing the levels of LC3B-II and p62 and quantitating GFP-LC3B by fluorescence microscopy. A luciferase reporter assay investigated the putative binding sites of miR-30b. The effects of miR-30b and antimiR-30b on autophagy, apoptosis and osteogenic differentiation of TNF-alpha-treated cells were determined by autophagosome, apoptosis and alkaline phosphatase assays, respectively. Results: TNF-alpha exposure decreased cell viability, increased apoptosis and positively regulated autophagy in ADTC5 cells. A direct interaction was detected between miR-30b and the mRNA 3'-UTRs of autophagy genes BECN1 and ATG5. Overexpression of miR-30b downregulated autophagy genes and upregulated pro-apoptotic gene expression in TNF-alpha-treated cells, while treatment with antimiR-30b had the inverse effect. Overexpression of miR-30b also downregulated ECM degradation and anti-miR-30b reverse TNF-alpha-induced ECM degradation. Conclusions: Anti-miR-30b enhanced autophagy and attenuated cartilage degradation and played a protective role in TNF-alpha-induced apoptosis of ATDC5 cells. Anti-miR- 30b may therefore elevate cellular survival during inflammation and has therapeutic potential for inflammatory diseases such as osteoarthritis. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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