期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 875, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2020.173057
关键词
Vorinostat; histonedeacetylaseinhibitor(HADCi); Cervicalcancer; majorhistocompatibilityclassI-relatedchainA(MICA); phosphoinositide3-kinases(PI3Ks)
资金
- Medical Project of Science and Technology Department of Foshan [2018AB000031]
- Science and Technology Bureau of Foshan [FS0AA-KJ218-1301-0008]
- Foshan anti-tumor innovative drug research and engineering technology center [FS0AA-KJ819-4901-0082]
- Foshan Medicine Dengfeng Project of China (2019-2021)
Vorinostat has good therapeutic efficacy against primary cutaneous T-cell lymphoma in the refractory stage. However, the molecular mechanism by which it inhibits solid tumors has not been clarified. To investigate the tumor inhibitory mechanism of vorinostat in cervical cancer, this study used Cell Counting Kit-8, flow cytometry, cell invasion and migration assays and the wound healing assay to evaluate the effects of vorinostat on cervical cancer cell proliferation, apoptosis, cell cycle, migration, and invasion. Real-time quantitative PCR and immunoblotting were used to detect gene and protein expression, respectively, of major histocompatibility class I-related chain A, phosphoinositide 3-kinase, phosphorylated PI3K p55 (Tyr199), and p-Akt (Ser473). The lactate dehydrogenase cytotoxicity assay was used to evaluate the ability of natural killer-92 cells to lyse cervical cancer cells. A xenograft nude mouse model was established to analyze the anti-tumor effect of vorinostat in vivo. Our results showed that vorinostat inhibited the proliferation, migration, and invasion of cervical cancer cells. Vorinostat also induced apoptosis and cell-cycle arrest in the S phase, inhibited PI3K (p110a), p-PI3K p55 (Tyr199), and p-Akt (Ser473) protein expression and upregulated MICA expression in vitro and in vivo, and promoted NK-92 cell-mediated cervical cancer cell lysis. The ability of vorinostat to upregulate MICA expression in cervical cancer cells was related to PI3K/Akt signaling. In brief, vorinostat upregulated MICA through the PI3K/Akt pathway and enhanced the sensitivity of cervical cancer cells to the NK cell-mediated cytolytic reaction. The results of this study demonstrate that vorinostat has anti-solid tumor effects on cervical cancer.
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