期刊
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
卷 47, 期 12, 页码 2765-2775出版社
SPRINGER
DOI: 10.1007/s00259-020-04784-0
关键词
ICAM-1; ImmunoPET; Companion diagnostics; Thyroid cancer; Melanoma
资金
- University of Wisconsin - Madison
- National Institutes of Health [P30CA014520]
- Natural Science Foundation of China [81771858]
Purpose Intercellular adhesion molecule-1 (ICAM-1, CD54) is an emerging therapeutic target for a variety of solid tumors including melanoma and anaplastic thyroid cancer (ATC). This study aims to develop an ICAM-1-targeted immuno-positron emission tomography (immunoPET) imaging strategy and assess its diagnostic value in melanoma and ATC models. Methods Flow cytometry was used to screen ICAM-1-positive melanoma and ATC cell lines. Melanoma and ATC models were established using A375 cell line and THJ-16T cell line, respectively. An ICAM-1-specific monoclonal antibody (R6-5-D6) and a nonspecific human IgG were radiolabeled with Cu-64 and the diagnostic efficacies were interrogated in tumor-bearing mouse models. Biodistribution and fluorescent imaging studies were performed to confirm the specificity of the ICAM-1-targeted imaging probes. Results ICAM-1 was strongly expressed on melanoma and advanced thyroid cancer cell lines. Cu-64-NOTA-ICAM-1 immunoPET imaging efficiently delineated A375 melanomas with a peak tumor uptake of 21.28 +/- 6.56 %ID/g (n = 5), significantly higher than that of Cu-64-NOTA-IgG (10.63 +/- 2.58 %ID/g, n = 3). Moreover, immunoPET imaging with Cu-64-NOTA-ICAM-1 efficiently visualized subcutaneous and orthotopic ATCs with high clarity and contrast. Fluorescent imaging with IRDye 800CW-ICAM-1 also visualized orthotopic ATCs and the tumor uptake could be blocked by the ICAM-1 parental antibody R6-5-D6, indicating the high specificity of the developed probe. Finally, blocking with the human IgG prolonged the circulation of the Cu-64-NOTA-ICAM-1 in R2G2 mice without compromising the tumor uptake. Conclusion ICAM-1-targeted immunoPET imaging could characterize ICAM-1 expression in melanoma and ATC, which holds promise for optimizing ICAM-1-targeted therapies in the future.
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