期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 54, 期 12, 页码 7366-7377出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.0c01601
关键词
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资金
- National Natural Science Foundation of China [41907285, 41771301]
- GDAS' Project of Science and Technology Development [2019GDASYL-0103053, 2019GDASYL-0301002, 2020GDASYL-20200402003]
- Science and Technology Planning Project of Guangzhou [202002020072]
- Guangdong Foundation for Program of Science and Technology Research [2019B121205006]
- Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01Z176]
- High-level Leading Talent Introduction Program of GDAS [2016GDASRC0103]
- Guangdong Introducing Innovative and Entrepreneurial Talents [2017GC010570]
Arsenite (As(III)) oxidation has important environmental implications by decreasing both the mobility and toxicity of As in the environment. Microbe-mediated nitrate-dependent As(III) oxidation (NDAO) may be an important process for As(III) oxidation in anoxic environments. Our current knowledge of nitrate-dependent As(III)-oxidizing bacteria (NDAB), however, is largely based on isolates, and thus, the diversity of NDAB may be underestimated. In this study, DNA-stable isotope probing (SIP) with C-13-labeled NaHCO3 as the sole carbon source, amplicon sequencing, and shotgun metagenomics were combined to identify NDAB and investigate their NDAO metabolism. As(III) oxidation was observed in the treatment amended with nitrate, while no obvious As(III) oxidation was observed without nitrate addition. The increase in the gene copies of aioA in the nitrate-amended treatment suggested that As(III) oxidation was mediated by microorganisms containing the aioA genes. Furthermore, diverse putative NDAB were identified in the As-contaminated soil cultures, such as Azoarcus, Rhodanobacter, Pseudomonas, and Burkholderiales-related bacteria. Metagenomic analysis further indicated that most of these putative NDAB contained genes for As(III) oxidation and nitrate reduction, confirming their roles in NDAO. The identification of novel putative NDAB expands current knowledge regarding the diversity of NDAB. The current study also suggests the proof of concept of using DNA-SIP to identify the slow-growing NDAB.
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