期刊
EMBO REPORTS
卷 21, 期 7, 页码 -出版社
WILEY
DOI: 10.15252/embr.201948425
关键词
Drosophila; Hedgehog; SAMD4; Smaug; Smoothened
资金
- NICHD
- ARC
- region Ile de France (SESAME)
- Paris-Diderot University
- Paris-Diderot University (ARS)
- CNRS
- University of Paris
- Fondation ARC pour la Recherche sur le Cancer [1112]
- ECOSSud
- l'Association Franco-argentine
- Fondation ARC pour la Recherche sur le Cancer
- CONICETArgentina
- Paris Sorbonne Cite
From fly to mammals, the Smaug/Samd4 family of prion-like RNA-binding proteins control gene expression by destabilizing and/or repressing the translation of numerous target transcripts. However, the regulation of its activity remains poorly understood. We show that Smaug's protein levels and mRNA repressive activity are downregulated by Hedgehog signaling in tissue culture cells. These effects rely on the interaction of Smaug with the G-protein coupled receptor Smoothened, which promotes the phosphorylation of Smaug by recruiting the kinase Fused. The activation of Fused and its binding to Smaug are sufficient to suppress its ability to form cytosolic bodies and to antagonize its negative effects on endogenous targets. Importantly, we demonstrate in vivo that HH reduces the levels of smaug mRNA and increases the level of several mRNAs downregulated by Smaug. Finally, we show that Smaug acts as a positive regulator of Hedgehog signaling during wing morphogenesis. These data constitute the first evidence for a post-translational regulation of Smaug and reveal that the fate of several mRNAs bound to Smaug is modulated by a major signaling pathway.
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