期刊
EMBO REPORTS
卷 21, 期 6, 页码 -出版社
WILEY
DOI: 10.15252/embr.201949942
关键词
G-quadruplex; lncRNAs; Myogenesis; RNA helicases; RNA-RNA interactions
资金
- EC-FP7 Marie Curie [ITN - 2013 GA 607720]
- ERC-2013 [AdG 340172-MUNCODD]
- H2020 - ERC-2019-SyG [855923-ASTRA]
- Telethon [GGP16213]
- AIRC [23053]
- PRIN 2017 [2017P352Z4]
Guanine-quadruplexes (G4) included in RNA molecules exert several functions in controlling gene expression at post-transcriptional level; however, the molecular mechanisms of G4-mediated regulation are still poorly understood. Here, we describe a regulatory circuitry operating in the early phases of murine muscle differentiation in which a long non-coding RNA (SMaRT) base pairs with a G4-containing mRNA (Mlx-gamma) and represses its translation by counteracting the activity of the DHX36 RNA helicase. The time-restricted, specific effect of lnc-SMaRT on the translation of Mlx-gamma isoform modulates the general subcellular localization of total MLX proteins, impacting on their transcriptional output and promoting proper myogenesis and mature myotube formation. Therefore, the circuitry made of lnc-SMaRT, Mlx-gamma, and DHX36 not only plays an important role in the control of myogenesis but also unravels a molecular mechanism where G4 structures and G4 unwinding activities are regulated in living cells.
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