期刊
CELLULAR & MOLECULAR BIOLOGY LETTERS
卷 21, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s11658-016-0027-8
关键词
Human bone-derived cells; Integrins; Osteoblast differentiation; Osteogenesis in vitro; Osteogenic markers; Primary osteoblasts
资金
- Polish funds for scientific research [N N302157037]
- Polish Ministry of Science and Higher Education [3 T08A 001 30]
Background: The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures. Methods: In this study, we isolated human bone-derived cells (HBDCs) and promoted their differentiation into osteoblasts. The following parameters were evaluated: cell number and viability, total protein expression, alkaline phosphatase activity, collagenous matrix production and osteogenic genes expression, i.e., gene coding for type I collagen and alkaline phosphatase. Results: It was proved the results show that HBDCs intensively proliferate during the first 7 days of culture followed by differentiation accompanied by an increase in alkaline phosphatase activity. Moreover, it was observed that during the differentiation of HBDCs, the expression of integrin beta 1 increased. Conclusions: The process was also accompanied by changes in cell shape and rearrangement of the actin cytoskeleton and focal contacts containing FAK and the integrin beta 1 subunit. We suggest that the beta 1 integrin subunit may be a suitable new target in studies of the differentiation of primary human osteoblasts in culture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据