期刊
CELL TRANSPLANTATION
卷 25, 期 5, 页码 913-927出版社
COGNIZANT COMMUNICATION CORP
DOI: 10.3727/096368915X689785
关键词
Mitochondria; Ischemic stroke; Mitochondria transfer; Neural protection
资金
- National Science Council of Taiwan [NSC 101-2811-B-005-015-MY3, 102-2628-B-005-007-MY3]
- Taichung Veterans General Hospital/National Chung Hsing University Joint Research Program [TCVGH-NCHU 1057606, 1027607]
- Ministry of Education, Taiwan, Republic of China, under the ATU plan
Transferring exogenous mitochondria has therapeutic effects on damaged heart, liver, and lung tissues. Whether this protective effect requires the symbiosis of exogenous mitochondria in host cells remains unknown. Here xenogenic mitochondria derived from a hamster cell line were applied to ischemic rat brains and rat primary cortical neurons. Isolated hamster mitochondria, either through local intracerebral or systemic infra-arterial injection, significantly restored the motor performance of brain-ischemic rats. The brain infarct area and neuronal cell death were both attenuated by the exogenous mitochondria. Although internalized mitochondria could be observed in neurons and astrocytes, the low efficacy of mitochondrial internalization could not completely account for the high rate of rescue of the treated neural cells. We further illustrated that disrupting electron transport or ATPase synthase in mitochondria significantly attenuated the protective effect, suggesting that intact respiratory activity is essential for the mitochondrial potency on neural protection. These results emphasize that nonsymbiotic extracellular mitochondria can provide an effective cell defense against acute injurious ischemic stress in the central nervous system.
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