期刊
CELL TRANSPLANTATION
卷 25, 期 8, 页码 1561-1574出版社
SAGE PUBLICATIONS INC
DOI: 10.3727/096368915X689776
关键词
Mesenchymal stem cells (MSCs); ROR2; Alveolar epithelium injury; Lipopolysaccharide (LPS)
资金
- National Natural Science Foundation of China [81070049, 81170057, 81201489, 81372093, 81300060, 81300043]
- Natural Science Foundation of Jiangsu Province of China [BK2011600, BK20131302]
- Graduate Innovation Project in Jiangsu Province of China [CXLX13_123, CXCL_151]
There are some limitations to the therapeutic effects of mesenchymal stem cells (MSCs) on acute respiratory distress syndrome (ARDS) due to their low engraftment and differentiation rates in lungs. We found previously that noncanonical Wnt5a signaling promoted the differentiation of mouse MSCs (mMSCs) into type II alveolar epithelial cells (AT II cells), conferred resistance to oxidative stress, and promoted migration of MSCs in vitro. As receptor tyrosine kinase-like orphan receptor 2 (ROR2) is an essential receptor for Wnt5a, it was reasonable to deduce that ROR2 might be one of the key molecules for the therapeutic effect of MSCs in ARDS. The mMSCs that stably overexpressed ROR2 or the green fluorescent protein (GFP) control were transplanted intratracheally into the ARDS mice [induced by intratracheal injection of lipopolysaccharide (LPS)]. The results showed that ROR2-overexpressing mMSCs led to more significant effects than the GFP controls, including the retention of the mMSCs in the lung, differentiation into AT II cells, improvement of alveolar epithelial permeability, improvement of acute LPS-induced pulmonary inflammation, and, finally, reduction of the pathological impairment of the lung tissue. In conclusion, MSCs that overexpress ROR2 could further improve MSC-mediated protection against epithelial impairment in ARDS.
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