期刊
DRUG AND CHEMICAL TOXICOLOGY
卷 45, 期 2, 页码 641-650出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/01480545.2020.1749652
关键词
Orthosiphon stamineus; HPLC; mitomycin C; peripheral blood lymphocytes; chromosomal aberration; sister chromatids exchange; serum deprivation; hydrogen peroxide scavenging; glutathione
资金
- Institute of Postgraduate Studies at the Universiti Sains Malaysia [PFM0028/13(R)]
- NKEA grant by Ministry of Agriculture Malaysia [304/CIPPM/650736/k123, RUT 1001/PFARMASI/851001]
- Universiti Sains Malaysia [RUT 1001/PFARMASI/851001, 304/FARMASI/6315201]
This study evaluated the cytoprotective, anti-mutagenic, and anticlastogenic activities of standardized Orthosiphon stamineus extract. The extract showed potent scavenging effects on hydrogen peroxide and other free radicals, stimulated glutathione production in liver cells, and exhibited powerful antimutagenic activities. Cytogenetic tests showed reduced mitotic and replicative indices without any chromosomal abnormalities or sister chromatid exchange. Pretreatment with the extract proved to be an efficient protocol in ameliorating the effects of mitomycin C.
Orthosiphon stamineus (O.S) is widely consumed for its medidcinal value including anti-inflammatory, anti-infective, and diuretic properties. The present study evaluates the cytoprotective, anti-mutagenic, and anticlastogenic efficacies of standardized extract of Orthosiphon stamineus. Normal liver cell line (WRL68) exposed to hydrogen peroxide and serum-deprived media as insults to evaluate cytoprotective and glutathione activation activities of (Et. O. s). Salmonella typhimurium TA98 and TA100 exposed to different concentrations of (Et. O. s). The influence of Et. O. s on mitotic, replicative indices as well as chromosomal aberration (CA) and sister chromatid exchange (SCE) induced in human peripheral blood lymphocytes by mitomycin C (MMC). The Et. O.s proved to be a potent scavenger for hydrogen peroxide and other free radicals in serum-depraved media, which showed to stimulate glutathione production in liver cells line. Moreover, it did not induce mutations in S. typhimurium subspecies TA98 and TA100. The standardized extract exhibited powerful antimutagenic activities as verified against both 2-nitrofluorene and sodium azide in S. typhimurium TA98 and TA100 cells, respectively. Cytogenetic tests showed high concentrations of Et. O. s to reduce the values of mitotic and replicative indices without any accompanying side effects, such as chromosomal abnormalities or SCE. To ameliorate MMC effects, pretreatment with the extract proofed to be efficient protocol. These data suggests that O. stamineus extract could be useful as cytoprotective, antimutagenic, and anticlastogenic efficacies, which owes to its potent chemoprevention, antioxidant, and glutathione activation properties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据