4.4 Article

Zinc Gluconate Induces Potentially Cancer Chemopreventive Activity in Barrett's Esophagus: A Phase 1 Pilot Study

期刊

DIGESTIVE DISEASES AND SCIENCES
卷 66, 期 4, 页码 1195-1211

出版社

SPRINGER
DOI: 10.1007/s10620-020-06319-x

关键词

Esophageal adenocarcinoma; Inflammation; Epithelial-to-mesenchymal transition; Chemoprevention; microRNA; Claudin

资金

  1. Sharpe Strumia Foundation
  2. NIH [R01CA118560]
  3. NIH Cancer Center Support Grant (CCSG) [P30 CA010815]

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This prospective study aimed to investigate the potential chemopreventive action of zinc in Barrett's esophagus (BE) by analyzing mRNA, miRNA, and protein changes in patients with BE who were given oral zinc gluconate. The results showed significant changes in gene and protein expression related to inflammation, epithelial-to-mesenchymal transition, and tumor suppression, suggesting a possible role of zinc in chemoprevention of esophageal cancer. Further prospective clinical trials are warranted to confirm these findings.
Background Chemopreventive effects of zinc for esophageal cancer have been well documented in animal models. This prospective study explores if a similar, potentially chemopreventive action can be seen in Barrett's esophagus (BE) in humans. Aims To determine if molecular evidence can be obtained potentially indicating zinc's chemopreventive action in Barrett's metaplasia. Methods Patients with a prior BE diagnosis were placed on oral zinc gluconate (14 days of 26.4 mg zinc BID) or a sodium gluconate placebo, prior to their surveillance endoscopy procedure. Biopsies of Barrett's mucosa were then obtained for miRNA and mRNA microarrays, or protein analyses. Results Zinc-induced mRNA changes were observed for a large number of transcripts. These included downregulation of transcripts encoding proinflammatory proteins (IL32, IL1 beta, IL15, IL7R, IL2R, IL15R, IL3R), upregulation of anti-inflammatory mediators (IL1RA), downregulation of transcripts mediating epithelial-to-mesenchymal transition (EMT) (LIF, MYB, LYN, MTA1, SRC, SNAIL1, and TWIST1), and upregulation of transcripts that oppose EMT (BMP7, MTSS1, TRIB3, GRHL1). miRNA arrays showed significant upregulation of seven miRs with tumor suppressor activity (-125b-5P, -132-3P, -548z, -551a, -504, -518, and -34a-5P). Of proteins analyzed by Western blot, increased expression of the pro-apoptotic protein, BAX, and the tight junctional protein, CLAUDIN-7, along with decreased expression of BCL-2 and VEGF-R2 were noteworthy. Conclusions When these mRNA, miRNA, and protein molecular data are considered collectively, a cancer chemopreventive action by zinc in Barrett's metaplasia may be possible for this precancerous esophageal tissue. These results and the extensive prior animal model studies argue for a future prospective clinical trial for this safe, easily-administered, and inexpensive micronutrient, that could determine if a chemopreventive action truly exists.

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