期刊
Cell Stem Cell
卷 19, 期 6, 页码 725-737出版社
CELL PRESS
DOI: 10.1016/j.stem.2016.08.009
关键词
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资金
- Italian Association for Cancer Research (AIRC)
- Astellas Foundation for Research on Metabolic Disorders fellowship
- Japan Society for the Promotion of Science fellowship
- Fondazione Citta della Speranza grant
- CARIPARO Bando Ricerca Pediatrica
- AIRC Special Program Molecular Clinical Oncology 5 per mille
- AIRC PI grant
- Epigenetics Flagship project CNR-Miur grants
- European Research Council (ERC) under the European Union [670126-DENOVOSTEM]
The ability to induce autologous tissue-specific stem cells in culture could have a variety of applications in regenerative medicine and disease modeling. Here we show that transient expression of exogenous YAP or its closely related paralogue TAZ in primary differentiated mouse cells can induce conversion to a tissue-specific stem/progenitor cell state. Differentiated mammary gland, neuronal, and pancreatic exocrine cells, identified using a combination of cell sorting and lineage tracing approaches, efficiently convert to proliferating cells with properties of stem/progenitor cells of their respective tissues after YAP induction. YAP-induced mammary stem/progenitor cells show molecular and functional properties similar to endogenous MaSCs, including organoid formation and mammary gland reconstitution after transplantation. Because YAP/TAZ function is also important for self-renewal of endogenous stem cells in culture, our findings have implications for understanding the molecular determinants of thesomatic stem cell state.
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