期刊
DEVELOPMENTAL CELL
卷 53, 期 4, 页码 473-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.04.009
关键词
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资金
- Canadian Institute for Health Research (CIHR) [153128]
- Krembil Foundation
- Strategic Priority Research Program of the Chinese Academy of Sciences grants [XDA16020601, XDB32010100]
- National Basic Research Program of China [2019YFA0110100, 2017YFA0102601, 2017YFA0103303]
- National Natural Science Foundation of China (NSFC) [91732301, 31671072, 31771140, 81891001]
- Beijing Brain Initiative of Beijing Municipal Science & Technology Commission [Z181100001518004]
- National Science Fund for Distinguished Young Scholars of China [81925009]
- National Natural Science Foundation of China [81790644]
- Research to Prevent Blindness
The development of single-cell RNA sequencing (scRNA-seq) has allowed high-resolution analysis of cell-type diversity and transcriptional networks controlling cell-fate specification. To identify the transcriptional networks governing human retinal development, we performed scRNA-seq analysis on 16 time points from developing retina as well as four early stages of retinal organoid differentiation. We identified evolutionarily conserved patterns of gene expression during retinal progenitor maturation and specification of all seven major retinal cell types, Furthermore, we identified gene-expression differences between developing macula and periphery and between distinct populations of horizontal cells. We also identified species-specific patterns of gene expression during human and mouse retinal development. Finally, we identified an unexpected role for ATOH7 expression in regulation of photoreceptor specification during late retinogenesis. These results provide a roadmap to future studies of human retinal development and may help guide the design of cell-based therapies for treating retinal dystrophies.
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