期刊
COLLOID AND POLYMER SCIENCE
卷 298, 期 7, 页码 707-717出版社
SPRINGER
DOI: 10.1007/s00396-020-04657-w
关键词
Self-assembly; Poly(ionic liquid) nanoparticles; Protein self-assembly; Cryo-electron microscopy; Single particle analysis; Cryo-electron tomography
资金
- Projekt DEAL
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [LU1423/1-1]
Cryo-electron microscopy (cryo-EM) is a powerful structure determination technique that is well-suited to the study of protein and polymer self-assembly in solution. In contrast to conventional transmission electron microscopy (TEM) sample preparation, which often times involves drying and staining, the frozen-hydrated sample preparation allows the specimens to be kept and imaged in a state closest to their native one. Here, we give a short overview of the basic principles of Cryo-EM and review our results on applying it to the study of different protein and polymer self-assembled nanostructures. More specifically, we show how we have applied cryo-electron tomography (cryo-ET) to visualize the internal morphology of self-assembled poly(ionic liquid) nanoparticles and cryo-EM single particle analysis (SPA) to determine the three-dimensional (3D) structures of artificial protein microtubules.
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