4.6 Article

AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21

期刊

CELL CYCLE
卷 15, 期 13, 页码 1733-1741

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2016.1183853

关键词

AS160/TBC1D4; cell cycle; cell proliferation; G1/S; p21

资金

  1. Chinese Academy of Sciences [292013312D11004]
  2. National Sciences Foundation of China [81270919]
  3. Sciences Foundation of Yunnan Province [39Y33H521261]

向作者/读者索取更多资源

AS160 (TBC1D4) has been implicated in multiple biological processes. However, the role and the mechanism of action of AS160 in the regulation of cell proliferation remain unclear. In this study, we demonstrated that AS160 knockdown led to blunted cell proliferation in multiple cell types, including fibroblasts and cancer cells. The results of cell cycle analysis showed that these cells were arrested in the G1 phase. Intriguingly, this inhibition of cell proliferation and the cell cycle arrest caused by AS160 depletion were glucose independent. Moreover, AS160 silencing led to a marked upregulation of the expression of the cyclin-dependent kinase inhibitor p21. Furthermore, whereas AS160 overexpression resulted in p21 downregulation and rescued the arrested cell cycle in AS160-depeleted cells, p21 silencing rescued the inhibited cell cycle and proliferation in the cells. Thus, our results demonstrated that AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle, and thereby revealed a molecular mechanism of AS160 modulation of cell cycle and proliferation that is of general physiological significance.

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