期刊
CELL BIOLOGY INTERNATIONAL
卷 41, 期 1, 页码 42-50出版社
WILEY-BLACKWELL
DOI: 10.1002/cbin.10698
关键词
caudal fin; crucian carp; Mitfa; pigment progenitor; Sox2
类别
资金
- National Science Foundation of China [31472272]
- National Basic Research Program of China [2012CB722305]
- Hunan Provincial Natural Science Foundation of China [14JJ2058]
- Hunan Provincial Education Department [15C0841]
- Hunan Provincial Innovation Foundation of Postgraduate [CX2015B172, CX2014B228]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [20134486]
Melanocyte stem cells are a population of immature cells which sustain the self-renewal and replenish the differentiated melanocytes. In this research, a light-colored region (LCR) is observed at the heel of caudal fin in juvenile crucian carp. By cutting off the caudal fin, the operated caudal fin can regenerate in accordance with the original pigment pattern from the retained LCR. As markers of stem cells, Oct4 and Sox2 have been found to be highly expressed in the LCR as well as Mitfa, a label of the melanoblasts. In vitro, Mitfa(+) melanoblasts are observed in the cells which are derived from the LCR and transfected with Mitfa-EGFP reporter by using Tol2 transposon system. Furthermore, by real-time qPCR, it is shown that the level of sox2 mRNA is gradually decreased from the LCR to proximal and distal caudal fin, and that of mitfa mRNA in the proximal caudal fin (PCF) is higher than that in the LCR, while it is the lowest in the distal caudal fin. Hence, we propose that the LCR is a pigment progenitor niche, sending melanocytes to the distal of caudal fin, which gradually emerges as caudal fin grow. We reveal that the LCR of caudal fin might be a niche of pigment progenitors, and contribute to pigment-producing stem cells in crucian carp.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据