期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 8, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13068-015-0395-8
关键词
Accessibility; Porosimetry; Tomography; Cellulose; Pretreatment; Biomass
资金
- Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio), an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0000997]
- BioEnergy Science Center (BESC)
- Office of Biological and Environmental Research in the US DOE Office of Science
Background: Substrate accessibility to catalysts has been a dominant theme in theories of biomass deconstruction. However, current methods of quantifying accessibility do not elucidate mechanisms for increased accessibility due to changes in microstructure following pretreatment. Results: We introduce methods for characterization of surface accessibility based on fine-scale microstructure of the plant cell wall as revealed by 3D electron tomography. These methods comprise a general framework, enabling analysis of image-based cell wall architecture using a flexible model of accessibility. We analyze corn stover cell walls, both native and after undergoing dilute acid pretreatment with and without a steam explosion process, as well as AFEX pretreatment. Conclusion: Image-based measures provide useful information about how much pretreatments are able to increase biomass surface accessibility to a wide range of catalyst sizes. We find a strong dependence on probe size when measuring surface accessibility, with a substantial decrease in biomass surface accessibility to probe sizes above 5-10 nm radius compared to smaller probes.
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