期刊
CHINESE CHEMICAL LETTERS
卷 31, 期 11, 页码 2897-2902出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2020.03.037
关键词
Vibration-induced emission; Autophagy; Viscosity; Fluorescence detection
资金
- National Natural Science Foundation of China [81672508, 61601218]
- Key University Science Research Project of Jiangsu Province [19KJA520005]
- Jiangsu Provincial Foundation for Distinguished Young Scholars [BK20170041]
- Natural Science Basic Research Program of Shaanxi [2019JM-016]
- China-Sweden Joint Mobility Project [51811530018]
- Fundamental Research Funds for the Central Universities
Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging. Herein, this study reported a novel mitochondria-targeted fluorescent probe DPACDY based on vibration-induced emission (VIE) for monitoring viscosity changes during mitochondrial autophagy. This probe contained N,N'-diphenyldihydrodibenzo[a,c]phenazine (DPAC) as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring. As the ambient viscosity increased, the vibration of DPAC-DY could be hindered, and subsequently resulting in the enhancement of fluorescence emission. In vitro and intracellular experiments indicated that the probe DPAC-DY showed highly sensitive response to viscosity due to VIE mechanism. Importantly, by virtue of this probe, in situ and real-time visualization of the specific viscosity dynamics during the mitochondrial autophagy process was achieved. Thus, this work provides a novel strategy for VIE-based viscosity response sensors applied to specific organelles and offers a platform for in-depth study of mitochondrial viscosity-related diseases. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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