期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 8, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13068-015-0324-x
关键词
(2S,3S)-Butane-2,3-diol; Metabolic engineering; alpha-Acetolactate synthase; meso-Butane-2,3-diol dehydrogenase
资金
- Chinese National Program for High Technology Research and Development [2011AA02A207, 2012AA022104]
- National Natural Science Foundation of China [31470164, 31400027, J1103515]
- Program for High Technology Research and Development of Shandong Province [2014GSF121030]
- Young Scholars Program of Shandong University [2015WLJH25]
Background: Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R, 3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R, 3R)-2,3-BD and meso-2,3-BD production. Results: The production of (2S, 3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the alpha-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-P-T7-budB-P-T7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose. Conclusions: The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.
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