期刊
CELL AND TISSUE RESEARCH
卷 368, 期 2, 页码 405-410出版社
SPRINGER
DOI: 10.1007/s00441-016-2427-5
关键词
Liver zonation; Liver polyploidy; Systems biology; Single molecule imaging; Stem cells
类别
资金
- Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics
- Leir Charitable Foundations
- Richard Jakubskind Laboratory of Systems Biology
- Lord Sieff of Brimpton Memorial Fund
- Human Frontiers Science Program
- I-CORE program of the Planning and Budgeting Committee
- Israel Science Foundation
- European Molecular Biology Organization Young Investigator Program
- European Research Council under European Union/ERC [335122]
- European Research Council (ERC) [335122] Funding Source: European Research Council (ERC)
The liver is a polyploid organ, consisting of hepatocytes with one or two nuclei each containing 2, 4, 8 or more haploid chromosome sets. The dynamic changes in the spatial distributions of polyploid classes across the liver lobule, its repeating anatomical unit, have not been characterized. Identifying these spatial patterns is important for understanding liver homeostatic and regenerative turnover, as well as potential division of labor among ploidy classes. Here, we use single molecule-based tissue imaging to reconstruct the spatial zonation profiles of liver polyploid classes in mice of different ages. We find that liver polyploidy proceeds in spatial waves, advancing more rapidly in the mid-lobule zone compared to the periportal and perivenous zones. We also measure the spatial zonation profiles of S-phase entry at different ages and identify more rapid S-phase entry in the mid-lobule zone at older ages. Our findings reveal fundamental features of liver spatial heterogeneity and highlight their dynamic changes during development and aging.
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