期刊
CELL BIOLOGY INTERNATIONAL
卷 44, 期 7, 页码 1491-1502出版社
WILEY
DOI: 10.1002/cbin.11344
关键词
fluid shear stress; osteogenesis; PIEZO1
类别
资金
- Key Research and Development Program of Shaanxi [2019SF-070]
- Fundamental Research Funds for the Central Universities [xzy022019043]
Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)-induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3-E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx-2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK-3 beta/beta-catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK-3 beta/beta-catenin pathway. Therefore, the results demonstrated that MC3T3-E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx-2 gene expression, partly through the AKT/GSK-3 beta/beta-catenin pathway.
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