期刊
CANCER CYTOPATHOLOGY
卷 128, 期 8, 页码 553-562出版社
WILEY
DOI: 10.1002/cncy.22278
关键词
CTC; FISH; indeterminate lung nodules; LDCT screening; lung cancer
资金
- SPORE grant [P50CA70907]
- Cancer Prevention Fellowship - National Cancer Institute [K07CA093592]
- National Cancer Institute [CA123208, CA55769]
- LungLife AI
- Thermo Fisher Scientific
Background Approximately one third of needle biopsies that are performed to rule out malignancy of indeterminate pulmonary nodules detected radiologically during lung cancer screening are negative, thus exposing cancer-free patients to risks of pneumothorax, bleeding, and infection. A noninvasive confirmatory tool (eg, liquid biopsy) is urgently needed in the lung cancer diagnosis setting to stratify patients who should receive biopsy versus those who should be monitored. Methods A novel antigen-independent, 4-color fluorescence in situ hybridization (FISH)-based method was developed to detect circulating tumor cells (CTCs) with abnormalities in gene copy numbers in mononuclear cell-enriched peripheral blood samples from patients with (n = 107) and without (n = 100) lung cancer. Results Identification of CTCs using FISH probes at 10q22.3/CEP10 and 3p22.1/3q29 detected lung cancer cases with 94.2% accuracy, 89% sensitivity, and 100% specificity compared with biopsy. Conclusion The high accuracy of this liquid biopsy method suggests that it may be used as a noninvasive decision tool to reduce the frequency of unnecessary needle biopsy in patients with benign pulmonary lesions.
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