期刊
CANADIAN JOURNAL OF MICROBIOLOGY
卷 66, 期 7, 页码 434-444出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/cjm-2019-0481
关键词
pneumolysin (Ply); mitochondrial damage; mtDNA; IFN-beta; epithelial cells
资金
- National Natural Science Foundation of China [31700804]
Pneumolysin (Ply) is a major virulence factor of Streptococcus pneumoniae. Ply-induced interferon-beta (IFN-beta) expression in host macrophages has been shown to be due to the accumulation of mitochondrial deoxyribonucleic acid (mtDNA) in the cytoplasm during S. pneumoniae infection. Our findings extend this work to show human bronchial epithelial cells that reside at the interface of inflammatory injury, BEAS-2B, adapt to local cues by altering mitochondrial states and releasing excess mtDNA. The results in this research showed that purified Ply induced the expression of IFN-beta in human epithelial cells, which was accompanied by mitochondrial damage both in vivo and in vitro. The observations also were supported by the increased mtDNA concentrations in the bronchial lavage fluid of mice infected with S. pneumoniae. In summary, our study demonstrated that Ply triggered the production of IFN-beta in epithelial cells, and this response was mediated by mtDNA released from Ply-damaged mitochondria. It displayed an impressive modulation of IFN-beta response to S. pneumoniae in epithelial cells.
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