期刊
BLOOD
卷 136, 期 7, 页码 845-856出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.2020004801
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资金
- National Institutes of Health (NIH) National Heart, Lung, and Blood Institute [R01HL091724]
- NIH National Institute of Allergy and Infectious Diseases [R21AI133261]
- NIH National Human Genome Research Institute [R01HG006130, R01 GM108716]
- Pennsylvania Department of Health
- NIH National Heart, Lung, and Blood Institute [T32HL007439]
- NIH National Institute of Diabetes and Digestive and Kidney Diseases [T32DK007780]
- NIH Eunice Kennedy Shriver National Institute of Child Health and Human Development [T32HD083185]
Hematopoietic stem and progenitor cells (HSPCs) in the bone marrow are derived from a small population of hemogenic endothelial (HE) cells located in the major arteries of the mammalian embryo. HE cells undergo an endothelial to hematopoietic cell transition, giving rise to HSPCs that accumulate in intra-arterial clusters (IAC) before colonizing the fetal liver. To examine the cell and molecular transitions between endothelial (E), HE, and IAC cells, and the heterogeneity of HSPCs within IACs, we profiled similar to 40 000 cells from the caudal arteries (dorsal aorta, umbilical, vitelline) of 9.5 days post coitus (dpc) to 11.5 dpc mouse embryos by single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing. We identified a continuous developmental trajectory from E to HE to IAC cells, with identifiable intermediate stages. The intermediate stage most proximal to HE, which we term pre-HE, is characterized by increased accessibility of chromatin enriched for SOX, FOX, GATA, and SMAD motifs. A developmental bottleneck separates pre-HE from HE, with RUNX1 dosage regulating the efficiency of the pre-HE to HE transition. A distal candidate Runx1 enhancer exhibits high chromatin accessibility specifically in pre-HE cells at the bottleneck, but loses accessibility thereafter. Distinct developmental trajectories within IAC cells result in 2 populations of CD45(+) HSPCs; an initial wave of lymphomyeloid-biased progenitors, followed by precursors of hematopoietic stem cells (pre-HSCs). This multiomics single-cell atlas significantly expands our understanding of pre-HSC ontogeny.
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