期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 117, 期 6, 页码 1724-1737出版社
WILEY
DOI: 10.1002/bit.27327
关键词
automated operations; high-throughput; laboratory adaptive evolution; microbial cultivation; microbial microdroplet culture system
资金
- National Key Scientific Instrument and Equipment Project of National Natural Science Foundation of China [21627812] Funding Source: Medline
- Tsinghua University Initiative Scientific Research Program [20161080108] Funding Source: Medline
- National Key Research and Development Program of China [2018YFA0901500] Funding Source: Medline
Conventional microbial cell cultivation techniques are typically labor intensive, low throughput, and poorlyparallelized, rendering them inefficient. The development of automated, modular microbial cell micro-cultivation systems, particularly those employing droplet microfluidics, have gained attention for their high-throughput, highly paralellized and efficient cultivation capabilities. Here, we report the development of a microbial microdroplet culture system (MMC), which is an integrated platform for automated, high-throughput cultivation and adaptive evolution of microorganisms. We demonstrated that the MMC yielded both accurate and reproducible results for the manipulation and detection of droplets. The superior performance of MMC for microbial cell cultivation was validated by comparing the growth curves of six microbial strains grown in MMC, conventional shake flasks or well plates. The highest incipient growth rate for all six microbial strains was achieved by using MMC. We also conducted an 18-day process of adaptive evolution of methanol-essential Escherichia coli strain in MMC and obtained two strains exhibiting higher growth rates compared with the parent strain. Our study demonstrates the power of MMC to provide an efficient and reliable approach for automated, high-throughput microbial cultivation and adaptive evolution.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据