期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 67, 期 4, 页码 527-535出版社
WILEY
DOI: 10.1002/bab.1924
关键词
Biocatalysis; Carbene transferase; De novo protein design
资金
- Biological and Biotechnological Sciences Research Council [BBI014063/1, BB/R016445/1, BB/M025624/1]
- Bristol Centre for Functional Nanomaterials (Engineering and Physical Sciences Research Council Doctoral Training Centre Grant) [EP/G036780/1]
- BBSRC [BB/R016445/1, BB/M025624/1] Funding Source: UKRI
The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-alpha-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarbenoid intermediate into the N-H sigma-bond to form a new N-C sigma-bond. Here we demonstrate that C45 can also catalyse N-H insertion reactions chemoselectively, even when the substrate contains an unprotected hydroxyl group.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据