期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 84, 期 7, 页码 1394-1400出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2020.1740970
关键词
Aluminum stress; soybean; antioxidant defense system
类别
资金
- Scientific Research Improvement Project of Research Fund for Young Scientists of BUA [SXQN201805]
- Beijing outstanding talent training for young backbone individual projects [2016000020124G049]
- Beijing Municipal Education Commission Research Project General Plan [KM201610020006]
This study investigated the antioxidant defense system involved in the tolerance of soybean (Glycine max) to aluminum (Al) stress. Physiological assays showed that the amount of superoxide free radicals (O-2(-)), hydrogen peroxide (H2O2), and malondialdehyde (MDA) were significantly lower in an Al-resistant soybean cultivar (cv. PI416937) than in an Al-sensitive soybean cultivar (cv. Huachun18). Comparative analysis of microarray data from both genotypes following Al-stress treatment revealed that the expression of a series of antioxidant enzymes genes was induced in the Al-resistant cultivar. The quantitative real time-PCR (qRT-PCR) assay showed that the transcript levels of genes encoding antioxidant enzymes, including GmCAT1, GmPOD1, GmGST1, GmAPX, GmGSH1, and GmSOD, were higher in the Al-resistant cultivar than in the Al-sensitive cultivar in Al-stress conditions. Furthermore, GmCAT1-overexpressing Arabidopsis plants had improved tolerance to Al-stress and lower O-2(-), H2O2, and MDA contents than wild-type plants. Therefore, providing evidence that the antioxidant defense system is essential for Al tolerance in soybean.
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