期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 84, 期 8, 页码 1685-1688出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2020.1756737
关键词
Cancer; lipophilic carbocyanine dye; lipid membrane; micronucleus; nuclear envelope
类别
资金
- Novartis Foundation Japan for the Promotion of Science [JP17H01878]
Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.
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