期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 112, 期 5, 页码 843-857出版社
WILEY
DOI: 10.1002/bit.25545
关键词
downstream bioprocessing; vaccines; gene therapy; process intensification; continuous purification
资金
- Sartorius Stedim Biotech
- Portuguese Science Foundation FCT-MCTES [SFRH/ BD/ 82032/ 2011, PTDC EBB-BIO/119501/2010, EXPL/ BBB-EBB/0790 2012]
- Fundação para a Ciência e a Tecnologia [PTDC/EBB-BIO/119501/2010, EXPL/BBB-EBB/0790/2012, SFRH/BD/82032/2011] Funding Source: FCT
The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral downstream purification processes, encompassing the classical unit operations and their recent developments. Emphasis is given to novel strategies for process intensification, such as continuous or semi-continuous systems based on multicolumn technology, opening up process efficiency. Process understanding in the light of the pharmaceutical quality by design (QbD) initiative is also discussed. Finally, an outlook of the upcoming breakthrough technologies is presented. Biotechnol. Bioeng. 2015;112: 843-857. (c) 2015 Wiley Periodicals, Inc.
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