4.3 Article

Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform

期刊

BIOMEDICAL MICRODEVICES
卷 22, 期 2, 页码 -

出版社

SPRINGER
DOI: 10.1007/s10544-020-00492-6

关键词

Microfluidic electrical impedance; Protein detection; Micro-bead assay; IL-6 and PCT; Point-of-care diagnostics; Sepsis

资金

  1. Center for Integration of Medicine and Innovative Technology Innovation (CIMIT)'s Point-of-Care Technology Center in Primary Care (POCTRN) Grant
  2. NIH [1 R21 AI146865 A]
  3. University of Illinois at Urbana-Champaign

向作者/读者索取更多资源

Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (similar to 10(3)pg/ml) and high (similar to 10(5)pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据