期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 112, 期 12, 页码 2611-2617出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.25685
关键词
lentivirus; gene therapy; HER2/neu
资金
- Chemical Engineering Department at Texas AM University
- National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1461705] Funding Source: National Science Foundation
Gene therapy represents a promising therapeutic paradigm for addressing many disorders, but the absence of a vector that can be robustly and reproducibly functionalized with cell-homing functionality to mediate the delivery of genetic cargo specifically to target cells following systemic administration has stood as a major impediment. In this study, a high-affinity protein-protein pair comprising a splicing-deficient naturally split intein was used as molecular Velcro to append a HER2/neu-binding protein (DARPin) onto the surface of a binding-deficient, fusion-competent lentivirus. HER2/neu-specific lentiviruses created using this in vitro pseudotyping approach were able to deliver their genetic reporter cargo specifically to cells that express the target receptor at high levels in a co-culture. We envision that the described technology could provide a powerful, broadly applicable platform for the incorporation of cell-targeting functionality onto viral vectors. Biotechnol. Bioeng. 2015;112: 2611-2617. (c) 2015 Wiley Periodicals, Inc.
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