期刊
BIOCONJUGATE CHEMISTRY
卷 31, 期 5, 页码 1545-1550出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.0c00197
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资金
- National Creative Research Initiative Grant through the National Research Foundation of Korea (NRF) - Korean Government (Ministry of Science ICT) [2014R1A3A2030423]
- Bio & Medical Technology Development Program through the National Research Foundation of Korea (NRF) - Korean Government (Ministry of Science ICT) [2012M3A9C4048780]
Herein, we report the use of two-photon fluorogenic probes using tetrazine-based bioorthogonal reactions with multicolor emissions that cover nearly all of the visible region. New fluorogenic probes were designed based on donor-acceptor-type naphthalene structures conjugated with a fluorescence-quenching tetrazine moiety for turn-on properties in one- and two-photon fluorescence. Our fluorescent probes showed a moderate to good turn-on ratio after bioorthogonal inverse electron demand Diels-Alder cycloaddition with trans-cyclooctenol in one- and two-photon fluorescence. We successfully applied our probes to mitochondria- and lysosome-selective bioorthogonal imaging in live cells with one-/two-photon and one-photon microscopy, respectively.
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