期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1862, 期 4, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2020.183193
关键词
Intramembrane proteases; Solubilization; Detergents; Liposomes; Amphipols; Lipid protein nanodiscs
资金
- Stichting Alzheimer Onderzoek
- Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen
- Regierende Burgermeister von Berlin-inkl. Wissenschaft und Forschung
- Bundesministerium fur Bildung und Forschung
- H2020 (Marie Curie Individual Fellowship)
Intramembrane proteases (IMPs) are proteolytic enzymes embedded in the lipid bilayer, where they cleave transmembrane substrates. The importance of IMPs relies on their role in a wide variety of cellular processes and diseases. In order to study the activity and function of IMPs, their purified form is often desired. The production of pure and active IMPs has proven to be a challenging task. This process unavoidably requires the use of solubilizing agents that will, to some extent, alter the native environment of these proteases. In this review we present the current solubilization and reconstitution techniques that have been applied to IMPs. In addition, we describe how these techniques had an influence on the activity and structural studies of IMPs, focusing on rhomboid proteases and gamma-secretase.
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