期刊
ARCHIVES OF MICROBIOLOGY
卷 202, 期 7, 页码 1729-1739出版社
SPRINGER
DOI: 10.1007/s00203-020-01884-1
关键词
Yeast; Hydrolysis; beta-Glucosidase; Cellobiase; Fermentation; Atlantic rain forest
类别
资金
- National Council for Scientific and Technological Development (CNPq) [454215/2014-2, 308627/2015-6, 308389/2019-0]
- Research and Innovation Support Foundation of Santa Catarina State (FAPESC) [749/2016, T.O. 2016TR2188]
- Support Program for Scientific and Technological Initiation from Federal University of Fronteira Sul (PRO-ICT/UFFS)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular beta-glucosidase activity [similar to 200 U (g cells)(-1) for 200 mM cellobiose and similar to 100 U (g cells)(-1) for 2 mM pNP beta G] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L-1 of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with K-m of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular beta-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.
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