Biochemical analysis of cellobiose catabolism in Candida pseudointermedia strains isolated from rotten wood

We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular beta-glucosidase activity [similar to 200 U (g cells)(-1) for 200 mM cellobiose and similar to 100 U (g cells)(-1) for 2 mM pNP beta G] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L-1 of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with K-m of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular beta-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.