Manipulation of AgNPs coated on selenium/carbonated hydroxyapatite/ε-polycaprolactone nano-fibrous via pulsed laser deposition for wound healing applications

Manipulation of nano-fibrous structures that imitate the normal extracellular matrix is one of the promising approaches that were suggested recently in wound healing biomedical applications. Silver nanoparticles (AgNPs) were deposited at different times of exposure on the surface of polycaprolactone (PCL) electrospun fibers containing carbonated hydroxyapatite (CHAP) which is substituted partially via selenite ions. This deposition was carried out using PLD technique. The yield compositions were investigated structurally and morphologically and were investigated also for their attachment towards the human osteoblast cell line (HFB4) after 3 days of in vitro culture. HFB4 cells lines have proliferated grow and spread through the fibrous compositions. Also antibacterial activity was carried out against S. aureus and E-coli. Surface roughness was enhanced with time coating enlarging, where roughness average increased from 27 at no coating to 35 nm after 20 min coating time. On the other hand, the antibacterial activity reached around 45.0% at t = 20.0 min against E. coli compared with the ciprofloxacin activity, while it recorded 43.0% against S. aureus. Increasing the coating time of nanoparticles for AgNPs@Se-CHAP/PCL enhances both cell growth and bacterial inhibition, this enhancement emphasizes that AgNPs@Se-CHAP/PCL is a dual function biomaterial that could be suggested as a potential material for wound healing applications.