期刊
APPLIED OPTICS
卷 59, 期 16, 页码 4833-4838出版社
OPTICAL SOC AMER
DOI: 10.1364/AO.389120
关键词
-
类别
资金
- Russian Science Foundation [17-75-30064]
- Russian Foundation forBasicResearch [18-32-00797]
- Russian Science Foundation [17-75-30064] Funding Source: Russian Science Foundation
We present a depth-localized illumination technique for wide-field fluorescence microscopy, based on long-range optical surface waves. This technique allows one to excite the fluorescence only in a thin near-substrate layer of the specimen. Our experimental setup is compatible with both upright and inverted microscopes. It provides fluorescent microscopic images, which are superior to the epifluorescence ones in signal-to-noise ratio, contrast, and detail. We demonstrate the applicability of our technique for imaging both bacterial and eukaryotic cells (E. coli and HeLa, respectively). (C) 2020 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据