期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 104, 期 13, 页码 5673-5688出版社
SPRINGER
DOI: 10.1007/s00253-020-10640-w
关键词
Recombinant therapeutic protein; Mammalian cell culture; Vector optimization; Cis-acting epigenetic regulatory elements
资金
- Basic Research Foundation of Key Scientific Research of Universities in Henan Province [20zx013]
- Major Science and Technology projects of Henan Province [191110311500]
- Key Scientific Research Projects in Universities of Henan Province [19A350008]
Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. One of the most promising strategies is expression vector cassette optimization based on the expression vector cassette. In this review paper, these approaches and developments are summarized, and the future strategy on the utilizing of expression cassettes for the production of recombinant therapeutic proteins in mammalian cells is discussed.
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