期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 104, 期 13, 页码 5619-5631出版社
SPRINGER
DOI: 10.1007/s00253-020-10635-7
关键词
alpha-l-Fucosidase; Fucosylated human milk oligosaccharides; Enzymatic synthesis; Trans-fucosylation
资金
- National Natural Science Foundation of China [31922073]
- Zhangjiagang international cooperation project [ZKH1905]
- National First-Class Discipline Program of Food Science and Technology of China [JUFSTR20180203]
alpha-l-Fucosidases (EC 3.2.1.51), catalyzing the hydrolysis of fucosides and/or the transfer of fucosyl residue, have been characterized and modified into a trans-fucosylation mode or, further, engineered to function as fucosynthase, which can be employed for the enzymatic synthesis of bioactive glycans, including fucosylated human milk oligosaccharides (HMOs). More than half of HMOs are fucosylated and have attracted ever-increasing interest because of their excellent physiological functions on breast-fed infants. To date, the characterization of novel fucosidases and molecular modification of these enzymes have been extensively studied to efficiently synthesize valuable fucosylated compounds. Herein, we discuss the advantages and challenges of different strategies for the production of HMOs and compare various donor/acceptor substrates used for the synthesis of fucosylated HMOs and their biomimetics. The implementation of trans-fucosylation patterns investigated in this paper via well-designed fucosidase mutants and proper reaction conditions may lead to development of an excellent platform, serving both fundamental studies and industrial-scale processes, for valuable carbohydrates synthesis.
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