期刊
CARDIOVASCULAR RESEARCH
卷 111, 期 1, 页码 66-73出版社
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvw072
关键词
Cardiomyocyte function; Microtechnology; Force-length relation
资金
- European Research Council [615170]
- Stichting voor Fundamenteel Onderzoek der Materie (FOM)
- Netherlands organization for scientific research (NWO, VIDI) [91711344]
- CVON-consortium grant (ARENA)
- 7th Framework Program of the European Union ('BIG-HEART') [241577]
- ICIN-Netherlands Heart Institute
- European Research Council (ERC) [615170] Funding Source: European Research Council (ERC)
A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force-length (FL) relations analogous to the pressure-volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. Here, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart.
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