4.2 Article

Filamin C: a novel component of the KCNE2 interactome during hypoxia

期刊

CARDIOVASCULAR JOURNAL OF AFRICA
卷 27, 期 1, 页码 4-11

出版社

CLINICS CARDIVE PUBL PTY LTD
DOI: 10.5830/CVJA-2015-049

关键词

LQTS; KCNE2; filamin C (FLNC); hypoxia; arrhythmia

资金

  1. National Research Foundation [FA2006040400017]

向作者/读者索取更多资源

Aim: KCNE2 encodes for the potassium voltage-gated channel, KCNE2. Mutations in KCNE2 have been associated with long-QT syndrome (LQTS). While KCNE2 has been extensively studied, the functions of its C-terminal domain remain inadequately described. Here, we aimed to elucidate the functions of this domain by identifying its protein interactors using yeast two-hybrid analysis. Methods. The C-terminal domain of KCNE2 was used as bait to screen a human cardiac eDNA library for putative interacting proteins. Co-localisation and co-immunoprecipitation analyses were used for verification. Results: Filamin C (FLNC) was identified as a putative interactor with KCNE2. FLNC and KCNE2 co-localised within the cell, however, a physical interaction was only observed under hypoxic conditions Conclusion: The identification of FLNC as a novel KCNE2 ligand not only enhances current understanding of ion channel function and regulation, but also provides valuable information about possible pathways likely to be involved in LQTS pathogenesis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据