期刊
ANIMAL BIOTECHNOLOGY
卷 32, 期 6, 页码 798-805出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2020.1752703
关键词
IGF-1; oocyte competence; embryo development; gene expression; ovine
资金
- ICAR-National Agricultural Science Fund [NFBSFARA/BSA-4005/2013-14]
The study showed that IGF-1 treatment significantly improved the developmental competence of ovine oocytes, enhancing the maturation rate and embryo development. This effect was achieved through the regulation of the PI3K/Akt and apoptosis signaling pathways.
This study aimed to assess the effect of the insulin-like grow factor 1 (IGF-1) treatment duringin vitromaturation on the gene expression and developmental ability of ovine oocytes. Ovine cumulus-oocyte complexes (COC) were maturedin vitrowithout (control) or with the supplementation of IGF-1 (100 ng/ml) and then subjected toin vitrofertilization and culture. The rate of oocyte maturation and embryo development was recorded and expression of the selected genes (involved in the PI3K/Akt and apoptosis signaling) was assessed in the matured oocytes. The IGF-1 treatment significantly (p < .05) improved the oocyte maturation rate (%) as compared to the control (81.5 +/- 2.40 vs. 73.6 +/- 0.94). Similarly, as compared to the control, the IGF-1 treatment significantly (p < .05) improved the rate (%) of cleavage (54.7 +/- 1.58 vs. 67.2 +/- 3.65) and the formation of 4-8 cell embryos (30.7 +/- 2.89 vs. 44.1 +/- 4.01) and morula (20.7 +/- 2.08 vs. 32.8 +/- 2.78). The IGF-1 treatment significantly (p < .05) upregulated the expression ofIGF1R,PI3KR1,AKT1andBCL2and downregulated the expression ofGSK3 beta,FOXO3andCASP9in the matured oocytes. In conclusion, the IGF-1 treatment significantly improved the developmental competence of ovine oocytes through the regulation of the PI3K/Akt and apoptosis signaling.
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