期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 26, 页码 10426-10430出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202002417
关键词
brain; electrochemistry; freely moving rats; ion channels; microelectrode arrays
资金
- NSFC [21635003, 21811540027]
- Innovation Program of Shanghai Municipal Education Commission [201701070005E00020]
Herein, we present an electrochemophysiological microarray (ECPM) for real-time mapping and simultaneous quantification of chemical signals for multiple ions in the deep brain of a freely moving rat, in which microelectrode arrays were developed for direct determination of multiple ions using open-circuit potentiometry. Specific recognition ionophores were synthesized and optimized for determination of K+, Ca2+, Na+ and pH. A reference electrode was also developed to avoid interferences in the brain. The microarrays were successfully applied in real-time monitoring and quantification of ions in a live brain. The extra current-free potentiometry allowed mapping and biosensing of chemical signals, together with recording of electrical signals in the whole brain without cross-talk, for the first time. Furthermore, the ECPM provided a platform for real-time monitoring of the dynamic changes of multiple ions in the deep brain of freely moving rat during a seizure.
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