期刊
ANALYTICAL CHEMISTRY
卷 92, 期 14, 页码 9699-9705出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c01032
关键词
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资金
- National Natural Science Foundation of China [31670035]
- Mega-projects of Science and Technology Research of China [2017ZX100102, 2018ZX10101-003-002]
- Public Service Development and Reform Pilot Project of Beijing Medical Research Institute [BMR2019-11]
- Military Medical Innovation Research Program [CX19015]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2M-1-008]
A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. A specific and rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay is a rapid detection method for several pathogens. Assays were performed within 5-15 min as a one-step single tube reaction at 39 degrees C. In this study, we established two RT-RAA assays for the S and orf1 ab gene of SARS-CoV-2 using clinical specimens for validation. The analytical sensitivity of the RT-RAA assay was 10 copies for the S and one copy for the orf1 ab gene per reaction. Cross-reactions were not observed with any of the other respiratory pathogens. A 100% agreement between the RT-RAA and real-time PCR assays was accomplished after testing 120 respiratory specimens. These results demonstrate that the proposed RT-RAA assay will be beneficial as it is a faster, more sensitive, and more specific tool for the detection of SARS-CoV-2.
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