期刊
ANALYTICAL CHEMISTRY
卷 92, 期 9, 页码 6727-6733出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c00808
关键词
-
资金
- National Natural Science Foundation of China [21904066]
- Fundamental Research Funds for the Central Universities [KJQN202056]
- National Key R&D Program of China [2017YFD0200506]
- State Key Laboratory of Fine Chemicals [KF1810]
- Innovation and Entrepreneurship Training Program for College Student of Jiangsu province [201910307050Y]
Heparin (Hep), widely used in clinics as an anticoagulant drug, has high degrees of heterogeneity and shares a similar disaccharide repeating unit with its GAG analogues. The development of reliable and convenient methods to discriminate Hep from its GAG analogues and detect trace GAG contaminants in Hep is meaningful for safe usage of Hep in clinics. Herein, five porphyrin-GO nanocomposites denoted as PP1-GO, PP2a-GO, PP2b-GO, PP3-GO, and PP4-GO were synthesized by assembling corresponding positively charged porphyrins onto the surface of GO. Controlled by a different number and position of the 4-N-methyl-pyridyl groups substituted at the porphyrins, these nanocomposites were determined to be cross-reactive toward Hep and other three commonly used GAGs including Chs, HA, and DS. A NIR sensor array PP-GO was thus constructed using these nanocomposites for GAGs discrimination and Hep quality control through pattern-based recognition. HCA and LDA calculated results indicated that PP-GO was powerful for discrimination of Hep and its GAG analogues in both PBS and even 10% serum media. Moreover, the PP-GO sensor array was successfully applied for the reliable discrimination of trace GAG contaminants in Hep with 100% accuracy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据