期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 412, 期 24, 页码 5981-5987出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02700-x
关键词
Chemical cross-linking; Cleavable cross-linkers; Electron microscopy; Mass spectrometry; Protein conformation; Protein-protein interactions; Structural biology
资金
- Projekt DEAL
- DFG [RTG 2467, 391498659]
- region of Saxony-Anhalt
Cross-linking/mass spectrometry (XL-MS) has come a long way. Originally, XL-MS was used to study relatively small, purified proteins. Meanwhile, it is employed to investigate protein-protein interactions on a proteome-wide level, giving snapshots of cellular processes. Currently, XL-MS is at the intersection of a multitude of workflows and the impact this technique has in addressing specific biological questions is steadily growing. This article is intended to give a bird's-eye view of the current status of XL-MS, the benefits of using MS-cleavable cross-linkers, and the challenges posed in the future development of this powerful technology. We also illustrate how XL-MS can deliver valuable structural insights into protein complexes when used in combination with other structural techniques, such as electron microscopy.
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