期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 412, 期 24, 页码 6101-6119出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02647-z
关键词
Electrophoresis; Electrochemical detection; Microdialysis; Nitric oxide; Neurotransmitters
资金
- National Institutes of Health [COBRE P20GM103638]
- University of Kansas School of Medicine Landon Center on Aging (NIH) [P30 AG035982]
- NIH Graduate Training Program in Dynamic Aspects of Chemical Biology Grant from the National Institutes of General Medical Sciences [T32GM008545]
- NSF [CHE-1411993]
- American Heart Association
- NIH [R21NS061202, R01NS042929]
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided. Graphical abstract
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